STA Core reagents are designed for mutation detection using TrimGen's proprietary STA technology. The application of STA Core reagents is flexible, and it allows customers to assemble custom test panels. Customers can use gene specific primers to test any mutation or SNP by combining STA core reagents.
Assay Method: Shifted Termination assay and fragment analysis
Assay Platform: Capillary Electrophoresis Sequencer (ABI Bioanalyzer 3100, 3700, 3130, 3500)
Assay Time: 2.5 - 3 hours
Features
- Accurately detect target nucleotide through multiple sequence specific steps
- Mutations are double-confirmed by both fragment size and fluorescent color
- Simultaneously detect any possible mutation of target nucleotide. For example "A" changed to C, G or T
- Multiplex test - Use fewer tubes and less sample DNA
- Easy Data Analysis - Clear-cut results show wild type and mutation peak only ("See the Peak, See the Mutation")
Reagent Sets Include
- Master Mix for DNA amplification
- C-UP Enzyme for PCR product clean up
- C-UP Buffer
- ST Reagent mix for STA reaction
- Sequencer loading buffer and size standard
Ordering Information
| Cat. No. | Product Name | Mutations Interrogated | Size | Documents |
| Cor-A | MutectorTM Core Reagents A | Universal reagents for detection of point mutations | 32 rxn | Product Flyer |
Cor-C | MutectorTM Core Reagents C | Universal reagents for detection of deletion, insertion, and translocations | 32 rxn | Product Flyer |
For research use only. Not for use in diagnostic procedures.
Example of detecting BRAF V600E mutation from FFPE samples:
Example of detecting JAK2 V617F mutation from FFPE samples:
Example of detecting KRAS codon 61 mutations from FFPE samples:
Example of detecting NRAS codon 12 mutations from FFPE samples:
Example of detecting CYP2D6 SNP (1846G>A, allele *4/*4) from blood samples: