The cytochrome P450 (CYP) family of enzymes performs the primary metabolism of many drugs in the human body. One of these CYP enzymes, CYP2D6, is responsible for the metabolism and elimination of approximately 25% of clinically used drugs1. The efficiency and amount of CYP2D6 enzyme varies significantly from person to person, which is the reason why some people eliminate CYP2D6-metabolized drugs quickly while others slowly. A drug's efficacy may be compromised if it is metabolized too quickly and a drug may become toxic if it is metabolized too slowly2. Thus, understanding the CYP2D6 variation is very important to adjust the dose of a drug metabolized by CYP2D6 for maximizing the efficacy and minimizing the toxicity.
The CYP2D6 gene has over 100 named alleles variability among the CYPs. The highly conserved pseudo genes show 90% similarity and make the assay more complicated.
TrimGen’s CYP2D6 genotyping assay has following features:
- Completely eliminated pseudo genes
- Simple protocol
- Clear, easy-to-read results in less than 4 hours
- Allele is double confirmed by peak color and fragment size
ASSAY INFORMATION
Target SNPs CYP2D6*2, *3, *4, *5, *6, *9, *10, *17, and *41
Assay Platform Capillary sequencer ABI 3100, 3700, 3130, 3500
Assay Format Two-tube test
Tube A includes alleles *2, *3, *4, *41, and 4180G>C
Tube B is includes alleles *6, *9, *10, and *17
*5 (gene deletion) show as no peaks (homozygous) or lower peaks (heterozygous)
Sample Required 100-200 ng DNA from whole blood or buccal swab
Assay Workflow
ORDER INFORMATION
| Cat. No. | Product Name | Target SNPs | Size | Documents |
| GP11 | CYP2D6 Genotyping Reagents | CYP2D6 *2, *3, *4, *5, *6, *9, *10, *17, *41, 4180G>C | 32 rxn | Manual, Product flyer |
For research use only. Not for use in diagnostic procedures.
DATA
Example Results of CYP2D6 Genotyping
Tube A detects 5 variations of CYP2D6 simultaneously in a single tube:
Tube B detects 4 variations of CYP2D6 simultaneously in a single tube:
Example of genotyping results (Tube A) - sample DNA is extracted from buccal swab using TrimGen's BuccalQuick kit:
Related Products
- Buccal Swab DNA Extraction – high yield DNA in 1 hour, 99% PCR success rate
References
- Wang B et al. (2009). New insights into the structural characteristics and functional relevance of the human cytochrome P450 2D6 enzyme". Drug Metab. Rev. 41:573
- Teh LK et al (2012). Pharmacogenomics of CYP2D6: molecular genetics, interethnic differences and clinical importance. Drug Metab. Pharmacokinet. 27: 55
- Llerena A et al. (2009). Pharmacogenetics of debrisoquine and its use as a marker for CYP2D6 hydroxylation capacity. Pharmacogenomics 10: 17–28.
- Lyon E et al. (2012). Laboratory testing of CYP2D6 alleles in relation to tamoxifen therapy. Genet Med. 14:990