WaxFreeTM Technology
WaxFreeTM is a specially designed kit for DNA or RNA extraction from FFPE (Formalin-fixed and paraffin-embedded) tissue.
• The kit uses a unique non-toxic de-paraffin method, which is safer for Lab operator.
• Compared to other methods, the kit shows more efficiency to release DNA/RNA from FFPE tissue, this high yield feature makes it successful for extracting the DNA/RNA from a tiny tissue section such as fine needle aspiration (FNA).
• The method has been validated by many downstream applications including PCR amplification, RT-PCR and NGS.
WaxFreeTM DNA Extraction Process and Example Results:
WaxFreeTM RNA Extraction Process and Example Results:
The technology uses a special chemistry and a resin and enzyme mix to remove inhibitors and release the nucleic acids into the extract. In a side-by-side comparison of 10 methods for nucleic acids extraction from FFPE samples, Okello et al. demonstrated that TrimGen'sWaxFreeTM method recovered the highest yields of amplifiable DNA1.
1. Okello, J.B., et al. (2010). Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues. Anal Biochem, 400:110.