Fragment analysis based BRAF mutation detection

Based on Shifted Termination Assay (STA) technology, our MutectorTM reagents contain special chemistry and a modified enzyme mixture to enrich the mutation signal 20-fold and detect as low as 1% mutation in wild type background. The MutectorTM reagents are widely used to detect BRAFKRAS and other mutations in clinical samples including FFPE (see STA/Mutector-related Publications).

The Difference

  • Detect more mutations in a single tube –   Simultaneously detect 5 mutations (see below) 
  • Use less clinical samples –  works for small amount of samples such as fine needle aspiration (FNA)
  • Detects low level (1-5%) mutations
  • Accurate –  like sequencing, the mutation is double confirmed by peak color and fragment size
  • Results in less than 4 hours

Assay Information

Assay Format            One tube test   
Target Mutations       Detects  V600E,  V600K,  V600R,  V600A,  V600G
Assay Platform         Capillary sequencer ABI 3100, 3700, 3130, 3500
Assay Sensitivity      Detects 1-2% BRAF mutations
Sample required       20-160 ng DNA 



Order Information

Cat. No. Product NameTarget Mutations SizeDocuments
GP02-EKBRAF mutation detection kitBRAF mutations in codon V60032 rxnManual

For research use only. Not for use in diagnostic procedures.


 The kit simultaneously detect 5 BRAF mutations in one tube:


Result from FFPE samples:

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