Based on Shifted Termination Assay (STA) technology, our MutectorTM reagents contain special chemistry and a modified enzyme mixture to enrich the mutation signal 20-fold and detect as low as 1% mutation in wild type background. The MutectorTM reagents are widely used to detect BRAF, KRAS and other mutations in clinical samples including FFPE (see STA/Mutector-related Publications).
The Difference
- Detect more mutations in a single tube – Simultaneously detect 5 mutations (see below)
- Use less clinical samples – works for small amount of samples such as fine needle aspiration (FNA)
- Detects low level (1-5%) mutations
- Accurate – like sequencing, the mutation is double confirmed by peak color and fragment size
- Results in less than 4 hours
Assay Information
Assay Format One tube test
Target Mutations Detects V600E, V600K, V600R, V600A, V600G
Assay Platform Capillary sequencer ABI 3100, 3700, 3130, 3500
Assay Sensitivity Detects 1-2% BRAF mutations
Sample required 20-160 ng DNA
Workflow
Order Information
| Cat. No. | Product Name | Target Mutations | Size | Documents |
| GP02-EK | BRAF mutation detection kit | 5 BRAF mutations in codon V600 | 32 rxn | Manual |
For research use only. Not for use in diagnostic procedures.
The kit simultaneously detect 5 BRAF mutations in one tube:
Result from FFPE samples:
Related Products
- FFPE Sample DNA Extraction– high yield DNA in 1 hour, 99% PCR success rate
- Mutation Analysis Reagents for Oncology – single platform for any somatic mutations
- Mutation Analysis Reagents for Pharmacogenetics – single platform for any genotyping