Oncology Mutation Analysis - STA Method

TrimGen's STA technology has been used to mutation analysis. The STA assay provides results in 3 hours with PCR-like sensitivity and sequencing-like accuracy. The STA mutation detection kits include all reagents and primer sets for the assay.  We also provide the STA core reagents kit that include the universal reagents for labeling and enriching mutation signal.  User can combine the core reagents kit with any selected gene primers to perform mutation analysis.

 

 
Assay Information
Assay method  Shifted Termination Assay (STA)
Sample required 10-100 ng DNA
Assay Time  3 hours
Instrument required

Thermal cycler
Capillary sequencer ABI 3100, 3700, 3130, 3500    

 

Mutation Detection Kits

Product NameCat. No.Targeted Mutations 
KRASGP05-C3KRAS Exon 2, Codons 12, 13
KRAS PlusGP06PKRAS Exon 3 & 4, Codons 61, 117, 146
NRASGP18NRAS exon 2, Codons 12, 13
NRAS PlusGP19PNRAS Exon 3 & 4, Codons 61, 117, 146
BRAFGP02-EKBRAF Exon 2, Codon V600
 POLEGP22P286R; S297F; V411L; P436R; A456P; S459F ; A465V
 MPLGP31Exon 10 S505N;W515L; W515K; W515A; W515R; W515S; W515K
 JAK2GP16V617P
 JAK2GP1724 deletions and insertion in Exon 12
 MYD88GP27L265P
 

The following assays are performed using STA core reagents kits

Product NameCat. No.Targeted Mutations 
EGFRCOR-AExon 18,  20, 21 point mutations
EGFRCOR-CDetect 120 deletions and insertion in Exon 19, 20 
PIK3CACOR-DExon 6, 9 point mutations
UGT1A1COR-CUGT1A1 promoter 7 TA repeats (UGT1A1*28)
FLT3COR-AExon 20  Internal tandem duplications (ITD)
NPM1COR-AExon 12 Insertions
IDH1 / IDH2COR-AExon 4  R132H, R132C, R132S, R132L, and R132G
ABL MutationsCOR-AM244V. Q252H. Y253F/H. E255K/V. T315I. M351T