| STA Core Reagents are set of reagents designed for mutation detection using Trimgen's proprietary STA technology. Each reagent has the flexibility to perform mutation detection with several gene-specific primer sets. For example STA Core Reagent A combined with a BRAF primer set can detect BRAF mutations, while the same reagents can be used for EGFR mutation detection when combined with an EGFR primer set. |
| |
 |
| |
| Assay Method:
Shifted Termination assay and fragment analysis. |
| |
| Assay platform:
Capillary Electrophoresis Sequencer |
| |
| Assay Time: 2.5 - 3 hours |
| |
| Features |
- Accurately detect target nucleotide through multiple sequence specific steps
- Mutations are double confirmed by both fragment size and fluorescent color.
- Simultaneously detect any possible mutations of target nucleotide. For example "A" changed to C, G or T.
- Multiplex test - Use fewer tubes and enable high throughput screening
- Easy Data Analysis - "See the Peak, See the Mutation"
|
Reagent Sets Include
|
- Master Mix for DNA amplification
- C-UP Enzyme for PCR product clean up
- C-UP Buffer
- ST Reagent mix for STA reaction
- Sequencer loading buffer and size standard
|
| |
| Ordering Information |
| Product |
Cat No |
Size |
Applications |
| STA Core Reagent A |
COR-A |
32 rxn |
BRAF, EGFR point mutations, KRAS codon 12 mutations |
| STA Core Reagent B |
COR-B |
32 rxn |
KRAS codon 13 mutations |
| STA Core Reagent C |
COR-C |
32 rxn |
EGFR deletions and insertions, Bcr-Abl |
| STA Core Reagent D |
COR-D |
32 rxn |
KRAS codon 61 mutations, PIK3CA mutations |
|
| For research use only. Not for use in diagnostic procedures. |
| |
| STA Core Reagent Flyer |
| |
