| TECHNOLOGIES |
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| Shifted Termination Assay (STA)
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An accurate and sensitive method using specially modified enzymes and nucleotides to detect and differentiate mutations commonly missed by sequencing. Compared to single base primer extension methods, such as SNAPShot, STA's multi-base extension not only increases signal strength but also the fragment size to create mutation peaks that are easily distinguished from wild type.
STA technology can detect mutations in multiple genes as well as different types of mutations (point mutations, deletions and insertions) in the same tube.
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STA Enriches Mutation Signal to Detect Low-level Mutations
The STA reaction enriches mutation signal by multi-base extension of target bases. Studies using a combination of wild type and mutant DNA show that the enrichment process enhances sensitivity, enabling the assay to detect mutations as low as 1%.
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Why Choose STA?
The STA reaction selectively extends detection primers with 1 to 20 nucleotides.
The primer extension is repeated multiple times to enrich the mutation signal.
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Assay Platform:
Capiliary electrophoresis (Sequencer) |
| Sequencer |
Software |
| Genetic analyzer 3100, 3130, 3700 | Data Collection Software v3.0 or v3.1, GeneMapper® V4.0, V4.1
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| Genetic analyzer 3500 |
Data Collection Software v1.0, GeneMapper® V4.1 |
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Shifted Termination Assay - Sequencer Platform |
