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PIK3CA mutation detection kit
Detects and differentiates 5 mutations in Exon 9 and 20 |
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| This single tube assay detects point mutations in the PIK3CA gene, including exon 9 codons E542 and E545 and exon 20 codon H1607. |
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Assay Method: Mutation enrichment by Shifted Termination Assay (STA)
and data collection by fragment analysis
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| Assay platform: Capillary Electrophoresis Sequencer |
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| Assay Time: 2.5 - 3 hours |
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| Assay Throughput: One 96-well plate is able to test 94 samples (including two controls). |
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| Mutations Detected: |
- Exon 9: E542K (G1624A), E545G (A1634G) and E545K.
- Exon 20: H1047R (A3140G) and H1047L (A3140T)
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